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KMID : 0603519980030020101
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Journal of Korean Association of Cancer Prevention
1998 Volume.3 No. 2 p.101 ~ p.106
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Transcriptional Inhibition of the Drosophila raf and PCNA Genes by the Homeodomain Protein Ftz and Engrailed
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Um Jae-Hee
Choi Tae-Yeong
Yoo Mi-Ae
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Abstract
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Raf-1 is a key molecule in many signaling pathways and is required for the regulation of cell proliferation and differentiation. It is also related to the tumorigenesis in some cancer cells. And proliferating cell nuclear antigen (PCNA) is required for the cellular DNA synthesis and cell cycle progression as an accessory protein of DNA polymerase δ. We have found that in the promoter region of D-raf, Drosophila homolog of human c-raf-1, there exist several putative homeodomain protein binding sites. Because in general, homeodomain proteins serve as transcription factors and regulate the expression of affected genes, we investigated the effect of homeodomain proteins on the expression of Drosophila raf and PCNA genes. Although the promoter region of PCNA gene used in this study has no consensus sites for the homeodomain protein binding, both genes were repressed by the homeodomain proteins, Ftz (Fushi tarazu) and Engrailed in cotransfection assays. These results suggest the possibility that the homeodomain proteins, Ftz and Engrailed, may inhibit cell proliferation through transcriptional inhibition of cell proliferation-related genes such as raf and PCNA.
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KEYWORD
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Drosophila, Raf, PCNA, Ftz, Engrailed, Cotransfection assay
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